abstract：:The biochemical reactions, carbohydrate content, and 16S-rRNA sequences of Tatlockia (Legionella) maceachernii and Tatlockia micdadei strains were studied. Except for catalase activity, Tatlockia strains were relatively inert in the biochemical tests commonly used in clinical laboratories. Phenotypically, the two Tatl...

journal_title：Canadian journal of microbiology

authors： Fox KF,Brown A

更新日期：1993-05-01 00:00:00

abstract：:Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks. We used pulsed-fi...

journal_title：Canadian journal of microbiology

authors： Buchrieser C,Brosch R,Catimel B,Rocourt J

更新日期：1993-04-01 00:00:00

abstract：:Polymerase chain reaction amplification was used to compare different regions of the cytochrome c3 gene from nine strains of Desulfovibrio vulgaris, to examine homology within the species. Six 30-base polymerase chain reaction primers and three probes were synthesized on the basis of the published nucleic acid sequenc...

journal_title：Canadian journal of microbiology

authors： Kwoh DY,Vedvick TS,McCue AF,Gevertz D

更新日期：1993-04-01 00:00:00

abstract：:A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly ...

journal_title：Canadian journal of microbiology

authors： Blaghen M,Vidon DJ,el Kebbaj MS

更新日期：1993-02-01 00:00:00

abstract：:An endo-beta-1,4-xylanase gene was cloned from Neocallimastix patriciarum 27 in the bacteriophage vector lambda gtWES lambda B and was subcloned into the plasmid vectors pUC18 and pUC19 in which xylanase activity was expressed in both orientations. The xylanase was located in the periplasmic space of the host, Escheri...

journal_title：Canadian journal of microbiology

authors： Lee JM,Hu Y,Zhu H,Cheng KJ,Krell PJ,Forsberg CW

更新日期：1993-01-01 00:00:00

abstract：:Studies of the molecular biology of human papillomavirus type 16 have been limited by the lack of a tissue culture system that is fully permissive for virus replication; as a result, high-titre stocks of infectious virus are not readily available. Therefore, studies of viral gene expression have relied on analysis of ...

journal_title：Canadian journal of microbiology

authors： Salloukh H,Lavery C,Smiley JR,Rawls WE

更新日期：1993-01-01 00:00:00

abstract：:To isolate thiamine excretors and (or) overproducers, 188 cultures belonging to nine yeast and three fungal genera were screened. Nine excreted thiamine as determined by both the presence of cross-feeding zones on a thiamine-free agar medium seeded with a thiamine-requiring yeast strain and by the direct detection of ...

journal_title：Canadian journal of microbiology

authors： Haj-Ahmad Y,Bilinski CA,Russell I,Stewart GG

更新日期：1992-11-01 00:00:00

abstract：:The oligosaccharides of human lactoferrin were enzymatically removed with glycopeptidase F, resulting in a preparation containing partial and fully deglycosylated human lactoferrin. The derivatives were separated by Concanavalin A affinity chromatography and compared with native human lactoferrin with respect to their...

journal_title：Canadian journal of microbiology

authors： Alcantara J,Padda JS,Schryvers AB

更新日期：1992-11-01 00:00:00

abstract：:We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation...

journal_title：Canadian journal of microbiology

authors： Nomoto K,Yokokura T,Nomoto K

更新日期：1992-08-01 00:00:00

abstract：:The binding of mutagenic pyrolyzates to cell fractions from some gram-negative intestinal bacteria and to thermally treated bacterial cells was investigated. 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) were effectively bound by several of the bacterial ce...

journal_title：Canadian journal of microbiology

authors： Zhang XB,Ohta Y

更新日期：1992-07-01 00:00:00

abstract：:Symbiotic nitrogen fixation may be limited by the transport of C4 dicarboxylates into bacteroids in the nodule for use as a carbon and energy source. In an attempt to increase dicarboxylate transport, a plasmid was constructed in which the Rhizobium meliloti structural transport gene dctA was fused to a tryptophan ope...

journal_title：Canadian journal of microbiology

authors： Rastogi V,Labes M,Finan T,Watson R

更新日期：1992-06-01 00:00:00

abstract：:The techniques in current use for detection of pathogens in environmental samples are restricted to those organisms whose replication in either culture media or cell culture is feasible. These methods lack the selectivity and sensitivity necessary for their unequivocal detection and identification. We have developed a...

journal_title：Canadian journal of microbiology

authors： Toranzos GA,Alvarez AJ

更新日期：1992-05-01 00:00:00

abstract：:Chitinases isolated from membrane and cytosolic fractions of two mucoraceous fungi, Choanephora cucurbitarum and Phascolomyces articulosus, were investigated. The membrane-bound chitinase was isolated by Bio-Gel P-100 and DEAE Bio-Gel A chromatographic techniques. On SDS-PAGE the chitinase from both fungi migrated as ...

journal_title：Canadian journal of microbiology

authors： Balasubramanian R,Manocha MS

更新日期：1992-04-01 00:00:00

abstract：:The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...

journal_title：Canadian journal of microbiology

authors： Gilchrist A,Fisher JA,Smit J

更新日期：1992-03-01 00:00:00

abstract：:Six effective Rhizobium leguminosarum bv. phaseoli strains were examined for nodulation competitiveness on common bean (Phaseolus vulgaris L.), using all possible two-strain combinations of inoculum. Nodule occupancy was determined with strain-specific fluorescent antibodies. The strains were divided into three groups...

journal_title：Canadian journal of microbiology

authors： George ML,Robert FM

更新日期：1992-02-01 00:00:00

abstract：:We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression ...

journal_title：Canadian journal of microbiology

authors： Batista S,Castro S,Aguilar OM,Martínez-Drets G

更新日期：1992-01-01 00:00:00

abstract：:The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on beta-glucuronidase activity, using the fluorogenic substrat...

journal_title：Canadian journal of microbiology

authors： Shadix LC,Rice EW

更新日期：1991-12-01 00:00:00

abstract：:A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurosp...

journal_title：Canadian journal of microbiology

authors： Chakraborty BN,Patterson NA,Kapoor M

更新日期：1991-11-01 00:00:00

abstract：:The susceptibility of congenitally immunodeficient mice to a nonencapsulated strain of Cryptococcus neoformans (strain M7) was evaluated. Gnotobiotic mice with defined congenital defects in innate immunity (beige) or cell-mediated immunity (athymic) or with combined defects in innate and cellular immunity (beige athym...

journal_title：Canadian journal of microbiology

authors： Salkowski CA,Balish E

更新日期：1991-11-01 00:00:00

abstract：:Antagonistic activity of the bacterium Pseudomonas cepacia against Trichoderma viride was greatly influenced by nutritional and environmental conditions. Xylose and trehalose strongly enhanced the antifungal activity of P. cepacia, whereas mannitol and glucose had little effect. The carbon sources that enhanced the an...

journal_title：Canadian journal of microbiology

authors： Upadhyay RS,Visintin L,Jayaswal RK

更新日期：1991-11-01 00:00:00

abstract：:Mutants of Trichoderma harzianum with altered antibiotic production were isolated using ultraviolet light mutagenesis. These included strains whose activity in a Fusarium oxysporum spore germination assay was greater than twice that of the parental strain and one that had no detectable antifungal activity. Characteris...

journal_title：Canadian journal of microbiology

authors： Graeme-Cook KA,Faull JL

更新日期：1991-09-01 00:00:00

abstract：:Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...

journal_title：Canadian journal of microbiology

authors： Shinagawa K,Omoe K,Matsusaka N,Sugii S

更新日期：1991-08-01 00:00:00

abstract：:Methanol extracts were obtained from L. interrogans serovars icterohaemorrhagiae and canicola and L. biflexa serovar patoc. Human sera from 167 normal individuals and 40 patients with different infectious diseases tested by complement fixation tests showed negative reactions. Sera from 100 patients with a suspicion of...

journal_title：Canadian journal of microbiology

authors： Koury MC,Cisalpino EO,Rangel Hde A

更新日期：1991-06-01 00:00:00

abstract：:Passive protective activities of three different classes of monoclonal antibodies in mice against challenge with strain ATCC 31432 (capsular type I) of Staphylococcus epidermidis were examined. Monoclonal IgM antibody passively protected mice against challenge with the homologous strain, whereas monoclonal IgG1 and Ig...

journal_title：Canadian journal of microbiology

authors： Ichiman Y,Usui Y,Suganuma M,Yoshida K

更新日期：1991-05-01 00:00:00

abstract：:The shikimate metabolites are key precursors to a large number of natural products, including aromatic amino acids. Chorismic acid is an important branch point in the biosynthetic pathway to aromatic amino acids. Chorismic acid is also unique among natural products since it is the only compound known to undergo an enz...

journal_title：Canadian journal of microbiology

authors： Zamir LO,Devor KA,Jensen RA,Tiberio R,Sauriol F,Mamer O

更新日期：1991-04-01 00:00:00

abstract：:Cellobiose transport by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes was measured using randomly tritiated cellobiose. When assayed at the same concentration (1 mM), total cellobiose uptake was one-fourth to one-third that of total glucose uptake. The abilities of F. succinogenes to transpo...

journal_title：Canadian journal of microbiology

authors： Maas LK,Glass TL

更新日期：1991-02-01 00:00:00

abstract：:Nodulation of soybeans by indigenous and inoculum strains of Bradyrhizobium japonicum was studied in field experiments in Wisconsin from 1983 to 86. Aqueous suspensions of bacteria were applied to seeds at the time of planting at levels of 7 × 10(7)-10(10) bacteria per 2.5-cm row. The predominant indigenous serogroup ...

journal_title：Canadian journal of microbiology

authors： McLoughlin TJ,Alt SG,Merlo PA

更新日期：1990-11-01 00:00:00

abstract：:The accumulation of radioactive Cs by fungi was studied by analysis of fruit bodies (n (total) = 205, n greater than or equal to 5 for 22 species) collected in 1988 in a Norwegian mountain area with high deposition of radiocaesium from the Chernobyl accident. To account for site variation, the radiocaesium content of ...

journal_title：Canadian journal of microbiology

authors： Bakken LR,Olsen RA

更新日期：1990-10-01 00:00:00

abstract：:Salmonella typhimurium and a series of rough lipopolysaccharide mutants derived from it were used as target bacteria to examine the antimicrobial capacity of magainin 2. Magainin 2 demonstrated a dose-related bactericidal activity against the smooth parent strain and the series of lipopolysaccharide mutants. The lipop...

journal_title：Canadian journal of microbiology

authors： Macias EA,Rana F,Blazyk J,Modrzakowski MC

更新日期：1990-08-01 00:00:00

abstract：:The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation pat...

journal_title：Canadian journal of microbiology

authors： Kudo H,Jakober KD,Phillippe RC,Cheng KJ,Barr DJ,Costerton JW

更新日期：1990-07-01 00:00:00

abstract：:Monoclonal antibodies reacting with the B subunit of Vibrio cholerae O1 strain 569B cholera toxin (CT-B) were used to identify unique and common epitopes of V. cholerae non-O1 and Vibrio mimicus CT-B. Vibrio cholerae non-O1 strains produced CT-B showing three monoclonal antibody reaction patterns (epitypes), which cor...

journal_title：Canadian journal of microbiology

authors： Tamplin ML,Jalali R,Ahmed MK,Colwell RR

更新日期：1990-06-01 00:00:00

abstract：:A nystatin-resistant mutant of Aspergillus niger is described that has quantitative changes in phospholipid composition compared with the wild type. The proportion of linoleic acid in total phospholipids was elevated in the mutant from 38 to 56% and the proportion of oleic acid was lowered from 28 to 12%. Amino acid t...

journal_title：Canadian journal of microbiology

authors： Mazumder C,Basu J,Kundu M,Chakrabarti P

更新日期：1990-06-01 00:00:00

abstract：:Of 24 bacterial species examined for lisinopril refractive peptidyl dipeptidase activity, only 8 contained activity. Activity in Pseudomonas maltophilia was more than fourfold higher than that of any other species. Pseudomonas maltophilia may be unique among bacteria in possessing high peptidyl dipeptidase activity th...

journal_title：Canadian journal of microbiology

authors： Stevens J,Fanburg BL,Lanzillo JJ

更新日期：1990-01-01 00:00:00

abstract：:The nucleotide sequence of the murE gene encoding the diaminopimelic acid adding enzyme of Escherichia coli is reported. The coding region consisted of 1413 base pairs and was separated from the ftsI (penicillin-binding protein 3) gene by 61 base pairs. The deduced primary structure of MurE comprised 471 amino acid re...

journal_title：Canadian journal of microbiology

authors： Tao JS,Ishiguro EE

更新日期：1989-11-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae Y222 was studied in the presence of the following iron-binding agents: Desferal, dipyridyl, and human and bovine transferrins. We report that cell growth and lanosterol 14 alpha-demethylase cytochrome P450 are not affected by Desferal but that dipyridyl and serum transferrins decreas...

journal_title：Canadian journal of microbiology

authors： Wright GD,Honek JF

更新日期：1989-10-01 00:00:00

abstract：:Characterization and enumeration of the adherent epimural community of the rumen wall of young, conventionally reared lambs were carried out from 2 to 21 days after birth. Three hundred strains were isolated by anaerobic procedures from three sites: dorsal, ventral, and caudal sacs, and from the sloughed epithelial ce...

journal_title：Canadian journal of microbiology

authors： Rieu F,Fonty G,Gouet P

更新日期：1989-07-01 00:00:00

abstract：:The effects of selected culture conditions on production of Shiga-like toxin-II variant by an edema disease strain of Escherichia coli (412) and E. coli TB1 (pCG6) containing the cloned genes for Shiga-like toxin-II variant were examined. Incubation time, culture media, incubation temperature, starting pH of the cultu...

journal_title：Canadian journal of microbiology

authors： MacLeod DL,Gyles CL

更新日期：1989-06-01 00:00:00

abstract：:The N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum strain Marburg has been purified with reasonable yield and much higher specific activity than previously reported. For the first time it has been shown that both N5,N10-methylenetetrahydromethanopterin dehydrogenase and...

journal_title：Canadian journal of microbiology

authors： Mukhopadhyay B,Daniels L

更新日期：1989-04-01 00:00:00

abstract：:Seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (IA and IB), two on day 4 (IVA and IVB), one on day 8 (VIIIA), and two on day 9 (IXA and IXB). The lambs were reared in these isolators until ...

journal_title：Canadian journal of microbiology

authors： Fonty G,Gouet P,Nebout JM

更新日期：1989-03-01 00:00:00

abstract：:The IncHII plasmid pHH1508a (208 kilobases) encodes resistance to potassium tellurite, trimethoprim, and streptomycin. Conjugative pili encoded by pHH1508a were isolated, purified, and used for preparation of anti-H pilus antiserum. Immuno-gold labelling experiments using H pilus specific antiserum showed that antigen...

journal_title：Canadian journal of microbiology

authors： Yan W,Taylor DE

更新日期：1989-02-01 00:00:00